UV MUTAGENESIS IN RADIATION-SENSITIVE STRAINS OF YEAST
نویسندگان
چکیده
منابع مشابه
UV mutagenesis in radiation-sensitive strains of yeast.
The yeast Saccharomyces cerevisiae appears to possess a single mutagenic or "error prony" pathway for the repair of UV damage; rev1, rev2, rev3 (Lemontt 1971a), rad6, rad8, rad9 and rad18 (Lawrence et al. 1974; present results). Strains carrying rad6 are the most sensitive to the lethal effects of UV light in this group and double mutants carrying rad6 and either rev1, rev3, rad9 or rad18 are n...
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The frequency of revertants induced by 60Co gamma rays of the ochre allele, cyc1-9, has been measured in radiation-sensitive strains carrying one of 19 nonallelic mutations and in wild-type strains. The results indicate that ionizing radiation mutagenesis depends on the activity of the RAD6 group of genes and that the gene functions employed are very similar, but probably not identical, to thos...
متن کاملEMS and UV mutagenesis in yeast.
Many fundamental biological processes have been elucidated by the isolation and analysis of mutants that are defective in such processes. Therefore, the methods to generate mutants are of great importance in model organisms. This unit describes two protocols for mutagenesis of yeast-using ethyl methanesulfate (EMS) and ultraviolet (UV) light. Each of these methods has been used successfully for...
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The role of the REV3 gene function in UV-induced mutagensis in the yeast Saccharomyces cerevisiae has been examined by determining the reversion of 12 well-defined cyc1 mutations in diploid strains homozygous for the rev3--1 or rev3--3 allele. The 12 cyc1 alleles include one ochre, one amber, four initiation, two proline missense, and four frameshift mutations. We find that the rev3 mutations r...
متن کاملIsolation of auxotrophic mutants of diploid industrial yeast strains after UV mutagenesis.
Auxotrophic mutants of the yeast Saccharomyces cerevisiae are usually isolated in haploid strains because the isolation of recessive mutations in diploids is thought to be difficult due to the presence of two sets of genes. We show here that auxotrophic mutants of diploid industrial sake yeast strains were routinely obtained by a standard mutant selection procedure following UV mutagenesis. We ...
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ژورنال
عنوان ژورنال: Genetics
سال: 1976
ISSN: 1943-2631
DOI: 10.1093/genetics/82.2.207